How To Use A Microscope Correctly, With An Operating Guide

We are all familiar with the microscope, and we have basically come into contact with it in the school laboratory. It can help us see things invisible to the human eye. It is a very practical laboratory instrument. But if you don't use it often, you still don't understand the correct use steps and overall structure. So the following is to facilitate everyone to understand the microscope more clearly.

How to use the microscope


(1) Take the lens and place it

   1. Hold the mirror arm with your right hand and the mirror holder with your left hand.

  2. Place the microscope on the experiment table, slightly to the left (the microscope is placed about 7 cm from the edge of the experiment table). Install the eyepiece and objective lens.

(2) Against the light

  3. Rotate the converter to align the low-power objective lens with the light hole (the front end of the objective lens and the stage should be kept at a distance of 2 cm).

  4. Align a larger aperture at the light hole. Look into the eyepiece with your left eye (open your right eye for easy drawing at the same time later). Rotate the reflector to reflect the light into the lens barrel through the light hole. Through the eyepiece, you can see the bright white field of vision.

(3) Observation

  5. Put the glass slide specimen to be observed (it can also be made of thin paper sheet printed with "6") on the stage, press it with the press clamp, and the specimen should be facing the center of the light hole.

  6. ​​Rotate the coarse collimation screw to slowly lower the lens barrel until the objective lens is close to the slide specimen (look at the objective lens to prevent the objective lens from touching the slide specimen).

  7. Look into the eyepiece with the left eye, and at the same time turn the coarse collimating screw in the opposite direction to make the lens barrel slowly rise until the object image is clearly seen. Turn the fine focus screw slightly to make the image of the object more clear.

   8. Use of high magnification objective lens: Before using high magnification objective lens, you must use low magnification objective lens to find the observed object, and adjust it to the center of the field of view, and then turn the converter to change the high magnification lens. After switching to a high-power lens, the brightness in the field of view becomes darker, so generally choose a larger aperture and use the concave surface of the mirror, and then adjust the fine collimation spiral. The number of objects to be viewed decreases, but the volume becomes larger.

(4) Organize

   8. After the experiment, wipe the surface of the microscope clean. Turn the converter, shift the two objective lenses to the sides, and slowly lower the lens barrel to the lowest point, and place the reflector vertically. Finally put the microscope into the mirror box and return it to its original place.

Structure of the microscope

The magnification of the microscope

   The magnification of a microscope is the eyepiece multiple times the objective lens multiple. If the eyepiece is 10 times and the objective lens is 40 times, the magnification is 40×10 times (400 times magnification). The limit magnification of the optical microscope is 2000 times, and it can distinguish two points separated by 1^10(-5) cm.

   After reading the above editor's explanation of the use of the microscope, the structure diagram and the magnification, how to use the microscope and what parts it is composed of, everyone should be more clear. According to the imaging principle, microscopes can be divided into two categories: optical microscopes and electron microscopes. The structure and function of each different microscope are also different. You must master its knowledge and use it correctly.

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