How to Choose a Microscope Correctly
In modern science and technology, microscope has turned into an essential tool, whose application fields and usage scenarios are enlarged and enriched without stop. For scientific researchers, it is an analytical instrument of general purpose for experiments and research and development; for schools, it is an outstanding teaching aid for probing into biology courses and propagating scientific knowledge; while for enthusiasts, it is a window to discover the novelty of the world. So, how to choose a suitable microscope in a correct way?
In the first place, choose the type of the microscope, then give consideration to the comfort of the operation experience, then choose the magnification, and finally take into account the quality of the optical system. Then let's take a closer look at it.
Ⅰ.Type of the microscope
Firstly, microscope can be chiefly classified into biological microscope, stereoscopic microscope, digital microscope, etc. based on the different applications observed.
- Biological microscope-characterized by large magnification times and small depth of field, it is suitable to use the biological microscope for observing biological cells, bacteria, culture of living tissue, fluid precipitation, etc., and besides, it can also be used to observe other transparent or semi-translucent objects, powders and fine particles.
- Stereoscopic microscope - characterized by small magnification times, large depth of field and three-dimensional sense of imaging, stereoscopic microscope is suitable for physical examination of objects or manipulation of observed objects, and it is also called "solid microscope" or "anatomical lens".
- Digital microscope- digital microscope, also referred to as video microscope, converts the physical images seen under the microscope into images on the microscope's own screen or computer through digital-to-analog conversion, which can be applied in teaching and scientific research and other related fields.
Besides, the suitable type of microscope for observing samples can be further judged by observing the corresponding charts at the following magnification of microscope.
The resolution ratio of a microscope is ultimately to satisfy the needs of visual resolution. The visual resolution is around 200μm, so the final magnification value of the microscope must be above 200μm, in order to satisfy the needs of visual resolution. If the resolution of the optical microscope indicated in the chart is 0.2μm, it would take a magnification of 1,000 times to bring the resolution of 0.2μm to 200μm for the human eye to see clearly.
Biological microscope is usually used to observe specimens made into slices, with magnification ranging from 4 times to 1,000 times. It is chiefly applied to observe tiny objects, such as bacteria and viruses, which can't be seen with naked eyes.
Stereoscopic microscope is used to observe objects in a direct way, chiefly by magnifying the surface details that are nearly invisible to human eyes, in a bid to see the target objects clearly. In comparison with a biological microscope, a stereoscopic microscope can achieve the observation results when it is magnified within 100 times.
Special observation requirements:
Q1: when observing samples, is it necessary to use a glass slide or a culture dish?
When the sample is placed on the glass slide for observation, it is necessary to select an upright biological microscope, and when a culture dish is used, it is required to select an inverted biological microscope.
Q2: is it required to take photos of microscopic mirror images or analyze and process images?
When necessary, it is suggested to choose a trinocular biological microscope, as well as a digital imaging system; if not required, a binocular biological microscope can be chosen.
Q3: are there any special requirements for the observation mode of the microscope?
Apart from bright-field observation (BF), if one or more requirements are raised for the microscope among dark field (DF), phase difference (PH), differential interference (DIC), relief phase contrast (RC), polarization (POL) and fluorescence (FL), please select the microscope with relevant functions.
III. Quality of Optical System
Objective lens plays a extremely crucial role in optical system. The higher the numerical aperture (NA) of objective lens, the stronger the resolution capability. Users are required to see the detail and budget, and choose the objective lens with appropriate numerical aperture (NA). Besides numerical aperture (NA), they are also required to evaluate the correction degree of spherical aberration and chromatic aberration of objective lens of the microscope. Choose the best objective lens within the budget. The quality of objective lens will directly influence the quality of data, so it is a highly significant factor to be brought into consideration.
Quite a number of microscope purchasers have asked us such a question, that is, "how much is a microscope? "or" how much is the quotation of a microscope of the XXX model", and generally, our answer is "what configuration do you need?" As a matter of fact, buying a microscope is similar to buying a computer to a large extent - everything should be configured at your request, such as how many observation modes (the number of objective lenses) are needed, whether software is required, etc., and these requirements have tremendously affected the price of the microscope.
V. Service Guarantee
Microscope belongs to a precision instrument, which requires to be backed up by specialized and high-quality services. Our SWIFT renders one-to-one exclusive customer services and one-year worry-free warranty. Our professional and effective after-sales service team can offer consumption experience to the satisfaction of customers.